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This was followed by a blocking step with 10% normal goat serum (NGS) in PBS for 1 h at room temperature to minimize non-specific binding

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First, the effectiveness of selenium supplementation is highly dependent on baseline selenium levels: reductions in autoantibody titers and improvements in mood or quality of life are more frequently observed in selenium-deficient regions or among individuals with low serum selenium, whereas trials conducted in selenium-replete populations often report limited or no benefit (123, 199201)

Dietary fiber is a crucial nutrient found in many plant-based foods, including fruits, vegetables, and legumes