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After 30 min of puromycin treatment, cells were lysed in RIPA Lysis and Extraction Buffer (Cat# 89901) that included Halt Protease Inhibitor Cocktail (Cat# 78430) and Halt Phosphatase Inhibitor Cocktail (Cat# 78420) from Thermo Fisher Scientific (Waltham, MA, USA)

Cascella R, Evangelisti E, Zampagni M, Becatti M, D'Adamio G, Goti A, Liguri G, Fiorillo C, Cecchi C

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