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Equal volumes of lysate or supernatant samples were loaded on NuPAGE 412% BisTris gels (Thermo Fisher Scientific, Waltham MA) without heating/boiling

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Marked improvement in objective function tests was achieved after three cycles of ozone therapy (Table IV)

For instance, docking studies can reveal how bioactive compounds such as CUR or PPR interact with key inflammatory mediators (e.g., NF-B, COX-2) or metabolic regulators (e.g., PPAR- , AMPK) (21, 120), offering mechanistic insights into their synergistic or antagonistic effects when combined with pharmaceuticals

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