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The GST tag can easily be removed following purification by addition of a protease if a suitable protease-cleavage site has been inserted between the GST-tag and the protein of interest (which is usually included in many commercially available sources of GST-tagged plasmids)

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J Nutr 142(3):441447 Daleprane JB, da Silva Freitas V, Pacheco A, Rudnicki M, Faine LA, Drr FA, Ikegaki M, Salazar LA, Ong TP, Abdalla DS (2012) Anti-atherogenic and anti-angiogenic activities of polyphenols from propolis