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The blots were washed again with TBST, and finally imaged with Amersham Imager 600 (GE Healthcare) after development with the Super Signal Sidurah Kit (Thermo Scientific)

Transfer to a dark bottle and refrigerate

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3.1.2 Ferroptosis-related lipid metabolism and RCC The acyl-CoA synthetase family member ACLS3 not only regulates the accumulation of lipid droplets in ccRCC and is essential for tumor growth, but also regulates sensitivity to ferroptosis in a manner dependent on the composition of exogenous fatty acids: both functions are implicated in the treatment of ccRCC (83)