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To construct the sorting vector pLKO.1-GST DAF for shRNA gene knockdown experiment, we amplified the coding sequence of N-signal-GST-EGFP-GPI DAF from the pEGFP-GST-GPI DAF plasmid and joined it with FastDigest KpnI/FastDigest BamHI (Thermo Fisher) linearized pLKO.1 vector using ClonExpress II One Step Cloning Kit

They compared the patients (good responders versus mild responders) who had already been taking vitamin B12 shots for at least six months to twenty years [17]

Neuroprotective effects of acetyl-l-carnitine (ALC) in a chronic MPTP-induced Parkinsons disease mouse model: endothelial and microglial effects

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The remaining animals continued on their diets as nulligravid controls