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Dihexa and Nle 1 -AnglV Induce Spinogenesis in Hippocampal Organotypic Cultures To further assess the physiological significance of the spine induction witnessed in dissociated neonatal hippocampal neurons the effects of dihexa and Nle '-AnglV on spine formation in organotypic hippocampal slice cultures was evaluated

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To ascertain substrate consumption and accumulation of metabolites, organic extracts of spent cultures were resolved by high-performance liquid chromatography (HPLC), equipped with a Shimadzu LC20-AT pump system, a diode array model SIL-M20A detector and a reversed-phase C18 column connected to a model SIL-20 A autosampler

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