RAW 264.7 cells were cultured in complete Dulbeccos modified Eagle medium (DMEM, Gibco) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS) (Avantor Seradigm), 2 mM L-glutamine (Gibco), 1 non-essential amino acids solution (Gibco) and 1 mM sodium pyruvate (Gibco) below 18 passages without exceeding 90% confluency
The Necessity of the Break: The Washout Period The break, or washout period, is as critical as the administration phase
Bravo R, Frank R, Blundell PA, & Macdonald-Bravo H
The lyophilized powder should be reconstituted with an appropriate research-grade solvent in a controlled laboratory environment prior to use in in vitro or preclinical assay systems
Your body is adapting to the peptide, fat mobilization is increasing, but visible changes take time