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For the construction of the metE mutant, the AES -which contained a Kan R cassette and the GFP gene optimized for its expression in mycobacteria (eGFP) (Supplementary Note 1)- was synthesized by GenScript

As a C-terminal fragment of human growth hormone with structural modifications, AOD-9604 represents a research subject in the broader field of peptide truncation chemistry specifically the use of short peptide fragments to study structure-activity relationships within larger parent proteins