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In summary, two main mechanisms of action of garlic organosulfur compounds emerged from the reported studies: (1) the reaction of garlic compounds to the free sulfhydryl group on the proteins and/or enzymes to inactivate them, and (2) the disruption of composition and integrity of bacterial cell membrane and/or cell wall
ROS are broadly defined as free radicals that contain unpaired electrons, such as superoxide (O 2 -) and hydroxyl radicals (OH), and reactive non-radicals that are oxidizing agents or are easily converted into radicals, such as hydrogen peroxide (H 2 O 2 ) [32]
It regenerates other antioxidants including Vitamins C and E, enhancing the overall antioxidant defense system
All the quantitative PCR (qPCR) analyses were run in triplicate, and results were expressed as fold changes calculated using the Ct method, as we published earlier (15, 41, 74, 76)