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Cells were washed with PBS and fixed with 4% paraformaldehyde (PFA) for 10 minutes at 4C, permeabilised for 15 seconds in a liquid nitrogen bath, blocked in 1% BSA / PBS for 1 hour at room temperature, incubated with D4H*-mCherry (1:25 in 1% BSA / PBS) for 2 hours, washed with PBS and post-fixed in 4% PFA for 10 minutes at room temperature

Key issues that need to be urgently addressed include: how to use the metabolic differences between T cell subsets (e.g., Tregs rely on FAO and CD8+ T cells rely on glycolysis) to design selective regulation strategies

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145 An analysis of 5-year outcomes for patients who received hypoglossal nerve stimulation found that 63% experienced a greater than 50% reduction in AHI, with sustained improvements in daytime sleepiness and overall quality of life