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The ROI was positioned so that one edge was placed at the periphery of the epidermis ensuring that the ROI extended over the epidermis and well into the dermis

2.2 Artificial cerebrospinal fluid (aCSF) composition The aCSF contains 124 mM NaCl, 3 mM KCl, 26 mM NaHCO 3 , 1.25 mM Na 2 HPO 4 , 1.8 mM MgSO 4 , 1.6 mM CaCl 2 , 10 mM glucose

OTHER INGREDIENTS: Microcrystalline Cellulose, Phosphatidylcholine 75%, Hydroxypropyl Beta cyclodextrin (HPCD), Phytosterols, Magnesium Stearate, Silica

Preparation and IV Access Prepare the solution under aseptic conditions