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The addition of the glucagon receptor agonist in retatrutide actively increases energy expenditure alongside appetite suppression, which is the primary mechanistic distinction and the basis for its stronger weight loss results in clinical trials

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DDP4 inhibitors (DPP4is) are commonly used to decrease blood levels of glucose and to treat T2DM

Mice were euthanized 12 to 14 hours post-hCG injection, and cumulus cells were removed enzymatically using 0.3% hyaluronidase in M2 medium