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Labeled IGF1 was separated from the free dye by size exclusion chromatography (Superdex 75 increase 10/300 GL, GE Healthcare) in the buffer containing 20 mM Hepes pH 7.5, 100 mM NaCl, and 2 mM EDTA

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This evidence base encompasses epidemiological investigations, occupational exposure studies, and biomarker-based research, collectively demonstrating measurable impacts on fertility parameters

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