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[DOI] [PMC free article] [PubMed] [Google Scholar] 115.Xiang L., Mou J., Shao B., Wei Y., Liang H., Takano N., Semenza G.L., Xie G

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To minimize the time that cells are kept in MAS buffer, just before calibration finished, the cell media was removed, and the cells were washed once with MAS (1) buffer before addition of 180 l MAS (1) buffer