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The ELISA plate was washed and blocked with I-BlockTM (Applied Biosystems) buffer (1 PBS + 0.2% I-Block + 0.05% Tween20) for 1 h at room temperature

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Those who often need additional iron include: Women of reproductive age who lose blood during menstruation Pregnant and breastfeeding women with increased nutrient needs Children and teenagers undergoing rapid growth Vegetarians and vegans who consume less heme iron Individuals recovering from surgery or blood loss Athletes who experience higher red blood cell turnover These groups can particularly benefit from learning how to take iron supplements properly to maintain balanced iron levels, support energy production, and prevent long-term deficiency symptoms

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