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Cells were grown at 37 C to an optical density at 600 nm (OD 600 ) of 0.70.8 in lysogeny broth (LPS solution LB-05) medium, and then 0.5 mM of isopropyl-1-thio--D-galactopyranoside (LPS solution IPTG025) was added

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There were no significant differences in GSH levels based on gender, skin type, melasma type, melasma pattern, and daily UV exposure

To avoid a possible effect of food and water intake and physical activity, the meal plan and total daily fluid intake as well as physical activeness were kept constant during the study and the patients received a dietary advice with provided by a renal dietitian before the study commenced