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The Arg3 substitution and N-terminal extension substantially reduce IGFBP-3 and IGFBP-5 binding affinity, making IGF-1 LR3 a preferred research tool over native IGF-1 for long-duration cell culture experiments where IGFBP-mediated sequestration would otherwise limit bioavailability

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Nomoto H, Oba-Yamamoto C, Takahashi Y, Takeuchi J, Nagai S, Yokoyama H, et al

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