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Our investigation employs the rat INS-1 832/3 -cell line, which naturally expresses the GLP-1R (26) and responds to GLP-1 by potentiating glucose-induced insulin secretion (GSIS) and increasing intracellular cAMP levels (19), making it a valuable model for studying the effect of GLP-1R phospho-site mutants on -cell signalling outputs

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The goal isnt just to feel an immediate energy rush after each shot, but to prevent levels from dropping back into a deficient range