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The foundational studies by Monnier, Schoenenberger, and colleagues who first isolated DSIP from rabbit brain venous blood during electrically-induced sleep demonstrated that intravenous or intraventricular administration of DSIP to rabbits and rats could induce behavioral and EEG changes consistent with sleep, particularly increases in slow-wave sleep

Indian Journal of Clinical Biochemistry , 32 , 134-141

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Histomorphometry added an interesting detail: Tb4-treated calluses were 23 percent smaller in overall size but contained 47 percent less old cortical bone

This helps ensure optimal benefits while avoiding excessive vitamin intake