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2.7 RNA extraction and qRT-PCR Quantitative real-time polymerase chain reaction (qRT-PCR) was carried out according to our previous published protocol ( GLP-1 and MPC2 expression levels were normalized to GAPDH and calculated via the 2 CT method

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In that regard, 3,3-dimethyl-1-butanol (DMB), a structural analog of choline has been found to inhibit microbial TMA lyase and reduced TMAO concentrations in mice fed with a choline or L-carnitine rich diet