semaglutide recombinant synthesis Method for preparing by biochemical method - Eureka METHOD FOR PREPARING SEMAGLUTIDE -

Two carboxyl groups on a side chain disclosed by patent CN111378028A are naked and are not protected by a protective group, the purity of an intermediate obtained in the subsequent coupling reaction with an Arg34GLP-1 (9-37) main peptide chain is not high, only 67.5 percent is obtained, the yield is seriously reduced, and the pH value in the reaction has a large influence on the side chain modification, so that various byproducts which are difficult to remove are easily generated, such as a double-modified impurity byproduct with both the 26 th position and the end position of the main peptide chain modified and a byproduct with the end position of the main peptide chain modified

4 showed that the purity of the compound 2 was 92.00%, and the residual amount of the raw material Arg34GLP-1 (9-37) was 0.89%

butoxycarbonyl (Boc) and the like

Features High Specificity Precisely cleaves at the Asp-Asp-Asp-Asp-Lys recognition site

Buffer: Sulphuric acid in water