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Individual colonies were expanded in 12 well plates, genomic DNA was extracted with PureLink Genomic DNA Mini Kit (Thermo Scientific, K182002), as well as protein lysates were prepared in RIPA buffer (50 mM Tris-Cl, pH 8, 150 mM NaCl, 5 mM EDTA, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, supplemented with protease inhibitors, Sigma, P8340)

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These amino acids function as building blocks for proteins

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