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doi: 10.1086/500092 135

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The 5% ICS is prepared in PBS containing Triton X-100/Tween-20

While both gene categories imply Ubx-RNA binding events coordinating splicing, two distinct mechanisms seem at play: the target genes regulated at both transcription and splicing levels largely depend on Ubx-DNA binding ability to regulate splicing, which cannot be dissociated from its RNA binding activity (Figure 1M)