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The optimal surfactant ratio was determined to reduce the CMC

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This effect is likely to be mediated through several pathways: a) Upregulation of antioxidant enzymes: The significant increase in catalase levels observed in the IF group suggests that IF enhances endogenous antioxidant defenses, as catalase is a key enzyme in detoxifying hydrogen peroxide and mitigating oxidative stress

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Although an excess of ROS may lead to a harmful effect on oocytes, the presence of ROS derived from mitochondrial respiration is necessary for certain cell signaling pathways involved in folliculogenesis, oocyte maturation, embryogenesis and implantation 58

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