Nuclear pellets were resuspended with IP buffer (150 mM NaCl, 50 mM Tris pH 7.5, 1 mM EDTA, 1% Triton), incubated on ice with vigorous regular vortex and sonicated (2x 30 seconds on/off, Picoruptor, Diagenode)
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(PubMed) Reinhart KM, Coleman CI, Teevan C, Vachhani P, White CM