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In an aspect of present invention, purification of semapeptide is carried out by precipitation at its isoelectric (pl) point

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in the second step, a tetralkylsilane bonded silica gel filler serves as a fixed phase, phosphoric acid serves as a mobile phase A, acetonitrile serves as a mobile phase B, and first HPLC (high performance liquid chromatography) purification is conducted so as to remove impurities of sermaglutide segment

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