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Establishment of baseline conditions For initial development of the process parameters, the Jung-Redmann (JR) peptide was chosen as a model sequence

0.33g of the crude Boc-His (Trt) -Aib-OSu and 1g of the compound 2 obtained in example 3 were dissolved in 20mL of N, N-dimethylformamide, 2mL of N, N-diisopropylethylamine was added, and the reaction was carried out at room temperature for 8 hours to obtain compound 4 with a purity of 74.08%, and a specific liquid phase diagram is shown in FIG

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the clarified supernatant fraction containing His6-AV-Ubiquitin-semapeptide was transferred to a beaker to load onto Ni-NTA resins while the pellet fraction containing cell debris was discarded

At the same time, the effect on weight management is also very prominent, especially for obese patients, which can significantly reduce their weight