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It performs a regulatory action on insulin phosphorylation and its impact on oxidative stress

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The Ponceau staining was then imaged on a Molecular Imager ChemiDocTM XRS+Imaging system (Bio-Rad) and destained by three washes in TBST (Tris-buffered saline (TBS, 25 mM Tris, 75 mM NaCl), with 0.5% (v/v) Tween-20) for 10 minutes each