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1.0pmol or 1.0nmol/2uL) was dissolved in 2.5% dimethyl sulfoxide ( DMSO ) and Dulbeccos Phosphate Buffered Saline ( DPBS ;VWR #020117)

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These observations were further confirmed by Hoechst 33342 staining, which revealed extensive detachment of cells from the cell culture substratum and increases in apoptotic bodies of HepG2 cells incubated with H 2 O 2 and TNF for 16 h (Fig