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DNA purification was carried out using SDSproteinase K digestion, phenol/chloroform extraction and ethanol precipitation

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At the same time, liver inflammation, intestinal flora displacement and concurrent infection caused by cirrhosis lead to the release of a large number of inflammatory factors and endotoxemia, causing renal microcirculation dysfunction, which can induce renal function injury ( Liver cirrhosis and primary liver cancer Primary liver cancer, as a high incidence of human malignant tumor, lacks typical clinical symptoms in the early stage, but the disease progresses rapidly, has a high risk of death, and the prognosis of patients is generally poor