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Nanocarrier uptake in midbrain organoids Control or PD organoids were embedded in 10 l Geltrex (Thermo Fischer Scientific) at the bottom of 24-well plates (1 organoid/well), then culture inserts were placed above them containing the BBB model as described in our previous study [24]

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Thus, we interpret the higher post-treatment values of serine, folate, homocysteine, and cysteine as the consequence of the greater entry of one-carbon units, hence, flux along the one-carbon cycle

The formula is: Dose Volume = Desired Dose divided by Concentration

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