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For LC-MS analysis, samples were thawed on ice-water bath, then 40 L of whole blood was injected into 450 L cold extraction solvent comprising of mixture of Acetonitrile:Methanol:Milli-Q water in ratio 40:40:20 according to protocol described in Presterud et al 35

This protection prevents oxidative stress that accelerates aging

All mice were housed in a normal environment and provided with food and water

The higher L-Glutamine dose (200mg) in Athlete Plus aids in muscle repair and GI barrier recovery after stress, especially for runners or military-style workouts

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