Companion satellite-cell cultures, prepared at the same time, were immunostained at 30-h post-plating with D3 anti-desmin mAb and DAB (a chromogenic HRP-substrate that forms an insoluble brown reaction product) to determine the percentage of myogenic cells present 18 ,19 cultures with less than 95% DAB-positive cells (namely, less than 95% desmin-positive myogenic purity) were not used for experiments just to ensure the reliability of data specific to myogenic cells in the employed primary cell culture systems
Recent findings suggest that omega-3 fatty acid, such as EPA and DHA, may act as indirect antioxidants in ASD
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Whenever possible, eat these foods raw or minimally heated, since heating and microwaving tend to destroy antioxidants
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