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The protein sample was extracted from membrane by adding a 2 solubilization buffer containing 20 mM HEPES pH 7.5, 500 mM NaCl, 2% (w/v) n-dodecyl-beta-D-maltopyranoside (DDM, Affymetrix), 0.4% (w/v) cholesteryl hemisuccinate (CHS, Sigma), and 2% (w/v) glycerol for 3 h at 4 C

single-nucleus RNA-seq indicates broad, cell-typespecific transcriptomic changes that overlap human AD signatures, and several of these effects are partly mediated by increased GSK3 activity, as pharmacologic GSK3 inhibition reverses Li-deficiency phenotypes

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Papers that used a nutrition-, metabolism- or inflammation-related stressor were excluded