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After 48 h, DMEM was replaced with Neurobasal medium (Gibco, 21103049) containing 50 B27 (Gibco, 17504044), 100 glutamine (Invitrogen, 25030081), and 100 penicillin-streptomycin (Thermo, 15140122)

Reviewing the team's patent and related documents at Talpos's request, Anna Mapp, the Edwin Vedejs Collegiate Professor of Chemistry at the University of Michigan, and also the President of the American Peptide Society, questioned the limited detail available and the absence of a published sequence for the peptide's parent protein, an omission she noted makes the original work impossible to reproduce

The selected GHK-Cu doses were calculated according to the concentration in human plasma ( The experimental procedures were performed complying with the Guide for the Care and Use of Laboratory Animals of China Medical University and approved by the Animal Care and Use Committee of China Medical University (Issue No

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Histologic recanalization occurred after 30 days with solution concentrations of 0.1% to 0.5%