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Using a microplate reader, absorbance from the supernatant of each sample was acquired and applied to estimate indicators of oxidative stress, including enzymatic antioxidants such as superoxide dismutase (SOD), glutathione (GSH), catalase (CAT), lipid peroxidation marker malondialdehyde (MDA) and protein content (PC) and nitric oxide (NO)

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nitric-oxide signaling

It is important to point out that the AFUA_6G09730/ gliF -AFUA_6G09740/ gliT intergenic sequence, which presumably functions as a divergent promoter, is only 302 bp long as annotated in the Af293 genome database