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Ms4a15 CRISPR homozygous mutations (30% efficiency) were generated in parental MF cells and validated by genotyping PCR and a 17-bp deletion in exon 2 by Tide (shinyapps.datacurators.nl/tide/)

The samples were analyzed on a Shimadzu UFLC system (Shimadzu Corporation, Kyoto, Japan) coupled to a QTRAP5500 (triple quadrupole linear ion trap mass spectrometer) equipped with a TurboIonSpray source (AB Sciex, Framingham, MA, USA)

These may include rash, pruritus, urticaria, and anaphylaxis