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Immediately following addition of the substrate, fluorescence was monitored using a M5 Spectramax plate reader using SoftMax Pro 5 at 37 C, excitation at 529 nm and emission at 600 nm, with a 590 nm cut-off filter

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Intra-VTA exendin-(9-39) pretreatment blocked the ability of CNO to attenuate cocaine seeking (Fig 3E & 3F) indicating that the suppressive effects of activating endogenous NTSVTA circuits on drug seeking depend, in part, on enhanced GLP-1 signaling in the midbrain

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