Cell culture Bone marrow-derived macrophages (BMDMs) were harvested from the femur and tibia of C57BL/6J mice and cultured in Dulbeccos modified Eagles medium (DMEM) containing 10% fetal bovine serum (FBS, Gibco, Carlsbad, CA, USA) and 10% L929 cell-conditioned medium, and maintained in a humidified incubator with 5% CO 2 at 37 C for 7 days [36]
doi: 10.3390/molecules29246026 68 AlamWRoccaCKhanHHussainYAschnerMDe BartoloAet al
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In bio-immunotherapy this therapeutic bio-modulation is aims to simulate specific leukaemia, adenocarcinoma or sarcoma regulatory codes, leading to cancer cure by forcing tumour cells back into healthy gene transcription, without apoptosis
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