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# 6592), and 1% penicillin/streptomycin solution (P/S

Quantification of the fluorescence emission by area Perimeters were drawn around the nuclei following the area marked with Hoechst, and the mean of nuclear CMFDA fluorescence was obtained (100 cells per condition in 3 separate experiments)

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The authors harvested nasal olfactory mucosa or olfactory bulb, and prepared frozen samples of that, demonstrating the pathway of nucleic acid transfer using the system, focusing on the major nose-to-brain pathways involving olfactory nerve and trigeminal nerve