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Preclinical Toxicology: Toxicity studies in rodents indicate that even high doses (e.g., 20 mg/kg) do not result in gross organ toxicity or lethality in the acute setting

doi: 10.1016/j.ejca.2017.10.008 87 LongoFPerriFPavoneEAversaCMaglioneMGGuidaAet al

In vitro , PPOs can be activated by, among others, the treatment with proteases, which was attempted for jr PPO1-wt using three commercial serine proteases, namely nagarse, trypsin and proteinase K

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Alkaloids contribute by activating Nrf2/HO-1 signaling, modulating the PINK1/Parkin pathway, and suppressing TLR4/NF-B and NLRP3 inflammasomes, while also upregulating Klotho protein levels [51]