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The placenta slides were put in NTMT solution (100 mM NaCl, 50 mM MgCl, 100 mM Tris pH 9.5, 10% Tween-20) 2 times for 10 min

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Metformin, commonly used to treat type II diabetes, has been shown to modulate gut microbiota composition, reducing the relative abundance of bacteria that encode choline TMA lyase, and thereby attenuating atherosclerosis [164]

doi:10.1186/2045-3701-4-29 Seale P (2015) Transcriptional regulatory circuits controlling brown fat development and activation