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Immunofluorescence staining of senescent cells Normal and DOX-induced senescent ATDC5 cells were fixed with 4% paraformaldehyde for 15 min, permeabilized with phosphate-buffered saline (PBS) containing 0.25% Triton X-100 for 10 min, and then blocked with 4% (v/v) BSA for 30 min at RT

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