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Each tube was then shaken at room temperature for 30 minutes prior to being centrifuged again at 13,500 rpm for 10 minutes

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Glucagon-like peptide 1: a potential anti-inflammatory pathway in obesity-related asthma

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Usually, the portal vein of such laboratory animals does not have culturable bacteria despite the presence of systemic sepsis, suggesting that BT takes place by transfer of intestinal bacteria predominantly into the lymphatic circulation via the intestinal lymph nodes, likely via the specialized M-cells overlying lymphoid aggregates [69, 70]