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Cells were pelleted at 300 g for 6 min, resuspended in complete MEM with 50 g ml 1 Gen at 2.5 10 5 cells per ml, and dispensed (80 l per well) into 384-well white and black plates (ThermoFisher) for luminescence and fluorescence measurements, respectively

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The molecular mechanisms accounting for the positive effect of LC on many physiological parameters in farm animals and poultry are not yet fully understood but many protective effects of LC reported in literature, have been postulated to be related to its antioxidant action

The bacA gene of Escherichia coli encodes an undecaprenyl pyrophosphate phosphatase activity

Vitamin E: an essential fat-soluble antioxidant that shields against free radicals, calms inflammation, and helps skin maintain moisture and elasticity