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Frozen cell pellets were thawed on ice, resuspended in 1.8 mL lysis buffer [50 mM potassium phosphate buffer, 1 mM dithiothreitol (DTT)

Mitral valve disease of the heart affects many small breeds especially Cavalier King Charles, Cocker Spaniels, Toy Poodles, Pomeranians and Yorkshire Terriers

Conversely, at higher weights above 8 mg, the excess material can lead to poor paste consistency, reduced conductivity, and slower electron transfer, which negatively affects the sensors responsiveness and stability

Vulliamy T, Kaeda J, Ait C

The presence of more than one autoantibody increased the statistical value of developing type 1 diabetes [1,3]