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Absence of lot analytical characterisation : a contaminated or mis-dosed lot can invalidate an entire experimental series Preregistration and ARRIVE 2.0 Modern animal research standards require protocol preregistration (OSF, PreclinicalTrials.eu) with ex ante definition of primary, secondary criteria and exclusion rules

This leads to a net increase in kisspeptin level and thus an increase in LH pulsatility, which is closely linked to the timing of hot flushes but is not causative (Figure 2)

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Greene, M

A common protocol for this stack involves daily injections for up to 30 days