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The beads were washed four times with a cold buffer containing 20 mM Tris, 150 mM NaCl, 1 mM DTT, 5% glycerol at pH 7.5, and then incubated with TEV protease overnight at 4 C to remove the 3FLAG tag

Furthermore, co-administration of beetroot extracts with doxorubicin, an effective but highly toxic chemotherapy drug, significantly reduces its cytotoxicity, probably by modulating the drugs induction of tumour promoting RONS [28,31]

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Avoid blood-thinning medications and supplements for 10-14 days before treatment, including vitamin E, aspirin, NSAIDs, fish oil, and garlic supplements

Increasing your fiber intake, which the Mediterranean diet naturally encourages, can provide some relief