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LCMS metabolomics analysis Sample preparation HCT116s cells (1 10 5 ) treated with or without 5 M 5FU were cultured in DMEM for 24 h and 72 h or supplemented with media containing uniformly labelled 13 C 6 -glucose (25 mM) for 6 h before sampling
For protocols emphasizing lipolysis and metabolic substrate shuttling, pairing with carnitine compounds is common in published stacks
Hyperglycemic stress and carbon stress in diabetic glucotoxicity
2020 Jun 1;9(6):1679
The full range of TB-500 benefits extends well beyond bone, but these skeletal effects are particularly relevant for fracture recovery
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